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Objective: To study the chemical composition of the stems and leaves of Cassiafloribunda. Methods: The chemical constituents from stems and leaves of C. floribunda were isolated by silica gel MCI, RP-18, TLC, and HPLC methods. Their structures were elucidated by spectroscopic methods and physicochemical properties. Results: Eighteen compounds were isolated from the 90% EtOH extract of C. floribunda and their structures were established ascassia cis-transdiphenylpropanoid(1), ethyl p-hydroxycinnamate (2), shonanin(3), dibutyl phthalate(4), 1,6,8-trihydroxyl-3-methyl-anthraquinone (5), 2,5-dimethyl-7-hydroxyl-chromogen, (6), 2-(2'-hydroxypropyl)-5-methyl-7-hydroxytryptophan(7), 4',7-dihydroxy-5-methoxy flavone(8), chrysoeriol(9), kaempferol(10), apigenin(11), 3-methoxy quercetin(12), 6-demethoxycapillarisin(13), 7,4'-dihydroxyflavone(14), luteolin(15), butin(16), liquiritigenin(17) anderiodictyol(18). Conclusion: Compound 1 is a new compound, and 2-18 are isolated from this plant for the first time.At the moment,2-4, 8, 9, 11, 13, 14, 16-18 are isolated from Cassia for the first time.
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Objective To study the chemical constituents of Zhuang medicine Cardiospermum halicacabum. Methods The compounds were isolated and purified by silica gel, polyamide gel, and Sephadex LH-20 chromatography. The structures of the compounds were identified on the basis of chemical and spectral methods. Results Thirteen compounds were isolated from the butanol extracts of C. halicacabum and identified as chrysoeriol-7-O-β-D-glucuronide butyl ester (1), chrysoeriol-7-O-β-D- glucuronide ethyl ester (2), chrysoeriol-7-O-β-D-glucuronide methyl ester (3), chrysoeriol (4), apigenin-7-O-β-D-glucuronide butyl ester (5), apigenin-7-O-β-D-glucuronide ethyl ester (6), apigenin-7-O-β-D-glucuronide methyl ester (7), apigenin-7-O-β-D- glucuronide (8), apigenin (9), luteolin-7-O-β-D-glucuronide methyl ester (10), luteolin-7-O-β-D-glucuronide (11), luteolin (12), and quercetin (13). Conclusion Compounds 1, 2, and 3 are new flavonoiels named as cardiosperoside D, cardiosperoside B, and cardiosperoside A. Compounds 5-8, 10, and 11 are isolated from this genus for the first time.
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AIM To establish an HPLC method for the simultaneous content determination of four constituents in Hydnocarpus anthelmintica Pierre.METHODS The analysis of 60％ ethanol extract from H.anthelmintica was carried out on a 35 ℃ thermostatic Diamonsil C18column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of 80％ acetonitrile-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient manner,and the detection wavelength was set at 347 nm.RESULTS Luteolin,hydnocarpusol,chrysoeriol and hydnocarpin showed good linear relationships within the ranges of 0.0523 6-1.047 μg (r =0.999 9),0.011 24-0.224 8 μg (r =0.999 9),0.029 46-0.589 2 μg (r =0.999 9) and 0.130 5-2.609 μg (r =0.999 9),whose average recoveries were 102.45％ (RSD=1.9％),98.66％ (RSD=1.8％),97.60％ (RSD=1.6％) and 97.88％ (RSD=1.4％),respectively.CONCLUSION This simple,accurate and sensitive method can be used for the quality control of H.anthelmintica.
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Objective: To study the chemical constituents from the root tubers of Fagopyrum dibotrys. Methods: The compounds were isolated and purified by means of chromatographic techniques and their structures were identified on the basis of spectral features. Results: Fourteen known compounds were isolated from methanol extract in the dry roots of F. dibotrys and thier structures were identified as luteolin (1), tricin (2), luteolin-7,4'-dimethyl ether (3), quercetin (4), genkwanin (5), chrysoeriol (6), protocatechuic acid (7), protocatechuic acid methyl ester (8), p-hydroxybenzaldehyde (9), glutinone (10), glutinol (11), olean-12-ene-3β, 7β, 15α, 28-tetraol (12), juglangenin A (13), and 21β-dihydroxy-olean-12-ene (14). Conclusion: Compounds 5 and 6 are firstly obtained from F. dibotrys. Compounds 12-14 are isolated from the plants of Fagopyrum Mill. for the first time.
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Objective: To study the flavonoids of the stems and leaves of male Trichosanthes kirilowii and to make a primary research on the structure activity relationship between flavonoids and their DPPH-scavenging capacity. Methods: Flavonoids from the stems and leaves of male T. kirilowii were separated by chromatographic techniques, such as polyamide resin, high-speed countercurrent chromatography and high performance liquid chromatography. According to the chemical properties and spectral analysis, the chemical structures of the compounds were identified. And we determined the antioxidant ability in vitro of seven flavonoids by DPPH methods. Results: Seven flavonoids were isolated from the stems and leaves of male T. kirilowii. They were luteolin (1), chrysoeriol (2), luteolin-7-O-β-D-glucoside (3), chrysoeriol-7-O-β-D-glucoside (4), apigenin-7-O-β-D-glucoside (5), diosmetin-7-O-β-D-glucoside (6), and quercetin-3-O-β-glucoside (7). Under the present experimental condition, the order of their DPPH-scavenging capacities was 1 > 3 > 7 > 2 > 4> 6 > 5. Conclusion: Compounds 1, 2, 6, and 7 are isolated from this part of male T. kirilowii for the first time. DPPH-scavenging capacities of the compounds 1, 3, and 7 are much stronger than others, but they all have 3',4' two adjacent hydroxide groups in B ring on the view of structure. DPPH-scavenging capacities of compounds 4 and 6 are much weaker than compound 3, but the former have 3' or 4' hydroxyl methylation in the structure. DPPH-scavenging capacity will also decrease if there is a 7 hydroxyl glycosylation in ring A by comparing compound 1 and 3. We speculate that it is caused by the increase of the steric hindrance.
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Objective: To investigate the chemical constituents from the leaves of Ananas comosus and their biological activities. Methods: The chemical constituents from the air-dried leaves of A. comosus were isolated and purified by the chromatography on silica gel and Sephadex LH-20 columns as well as recrystallization. Their structures were identified on the basis of physiochemical properties and spectroscopic data analyses, and the antibacterial activity and artemia lethal activity of the compounds were determined. Results: Eight compounds were isolated from the 95% ethanol extract in the leaves of A. comosus and identified as tricin-4′-O-[10″-O-(8″-hydroxyl) feruloyl-(9‴-O-p-coumaroyl) glyceryl] ether (1), 2, 4-dichlorobenzoic acid (2), tricin (3), chrysoeriol (4), 1-O-p-coumaroylglycerol (5), 1-O-feruloylglycerol (6), 1-O-feruloyl-3-O-p-coumaroyl-glycerol (7), and 1, 3-O-diferuloylglycerol (8). Compound 1 exhibited as well inhibitory activities as positive control Ciprofloxacin (CPFX) against Staphylococcus aureus and Escherichia coli, with MIC values of 0.156 μg/mL. The results of artemia lethal activity showed that the IC50 of the compounds 1 and 4 were 21.4 and 25.0 μg/mL, respectively. Conclusion: All the compounds except 5 are isolated from this plant for the first time. Among them, compound 1 is a new one and named ananasin A, which has the good antibacterial and artemia lethal activity.
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Objective: To study the chemical constituents from the radix and rhizome of Myrsine stolonifera. Methods: The chemical constituents were isolated and purified by D101 macroporous adsorption resin, silica gel, and Sephadex LH-20 column chromatography. The structures of the isolated compounds were identified on the basis of physicochemical properties and spectroscopic methods including 1H-NMR, 13C-NMR, and MS spectra. Results: Eleven compounds were identified as askaempferol (1), dihydrokaempferol (2), quercetin (3), 5, 7, 4'-trihydroxyisoflavone (4), chrysoeriol (5), quercetin-7-O-α-D-glucoside (6), (-)- epicatechin (7), 3, 5-dimethoxy-benzylalcohol-4-O-β-D-glucopyranoside (8), 2, 6-dimethoxy-4-hydroxyphenol-1-O-β-D- glucopyranoside (9), syringing (10), and (+)-lyoniresinol3α-O-β-D-glucopyranoside (11). Conclusion: Compounds 2, 4-11 are all isolated from the plants of Myrsine Linn. for the first time, and compounds 1-11 are all isolated from this plant for the first time.
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Objective: To study the chemical constituents from the stems and leaves of Cryptolepis buchananii. Methods: Compounds were isolated and purified by chromatography on silica gel, Sephadex LH-20, ODS columns, and preparative HPLC. Their structures were identified by NMR and MS, as well as comparison on spectral data with literature values. Results: Thirteen compounds were obtained from the EtOAc fraction of methanol extract in the stems and leaves of C. buchananii and their structures were elucidated as isoscopoletin (1), (+)-3-hydroxy-β-ionone (2), (3R, 6R, 7E)-3-hydroxy-4, 7-megastigmadien-9-one (3), ficusic acid (4), (+)-pinoresinol (5), (+)-8-hydroxypinoresinol (6), (+)-syringaresinol (7), diaaurantiamide acetate (8), loliolide (9), (-)-balanophonin (10), chrysoeriol (11), 9-hydroxy-10E, 12Z-octadecadienoic acid methyl ester (12), and ficusesquilignan A (13). Conclusion: All the compounds are isolated from the plants of Cryptolepis R. Br. for the first time.
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Objective: To study the chemical constituents from Scilla scilloides. Methods: The chemical constituents were isolated and purified by column chromatography and their structures were identified by spectral data. Results: Eighteen compounds were isolated from S. scilloides. Their structures were identified as scillascillin (1), 2-hydroxy-7-O-methylscillascillin (2), 4'-demethyleucomin (3), 5-hydroxy-7-methoxy-3-(4-hydroxybenzylidene) chroman-4-one (4), 4'-demethyl-3, 9-dihydroeucomin (5), 3'-hydroxy-3, 9-dihydroeucomin (6), 8-O-demethyl-7-O-methyl-3, 9-dihydropunctatin (7), apigenin (8), luteolin (9), chrysoeriol (10), 3-dehydro-15-deoxoeucosterol (11), 15-deoxoeucosterol (12), 4-allylpyrocatechol (13), norlichexanthone (14), drimiopsin C (15), 6-feruloylcatalpol (16), catalposide (17), and specioside (18). Conclusion: Iridoids (16-18) are isolated from the genus Scilla L. for the first time. Compounds 4, 7-10, and 13-18 are isolated from the plants of this genus for the first time.
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Objective: To study the chemical constituents of Artemisia anomala. Methods: Various chromatographic techniques were adopted to separate the constituents, and the spectroscopic analysis was used to identify their structures. Results: Seven compounds were isolated and identified as (4aS, 7S, 7aR)-methyl 7-hydroxy-7-methyl-1, 4a, 5, 6, 7, 7a-hexahydrocyclopenta [c] pyran-4- carboxylate (1), rehmaglutin D (2), (E)-6-hydroxy-2, 6-dimethylocta-2, 7-dienoic acid (3), chrysoeriol (4), luteolin (5), apigenin (6), and p-coumaric acid (7). Conclusion: Compound 1 is a new compound named artanoiridoid; Compound 2 is firstly reported in this genus; Compounds 3, 4, and 7 are separated from this plant for the first time.